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Detection and Quantification of Viable and Nonviable Trypanosoma cruzi Parasites by a Propidium Monoazide Real-Time Polymerase Chain Reaction Assay

Molecular techniques based on real-time polymerase chain reaction (qPCR) allow the detection and quantification of DNA but are unable to distinguish between signals from dead or live cells. Because of the lack of simple techniques to differentiate between viable and nonviable cells, the aim of this...

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Dades bibliogràfiques
Publicat a:Am J Trop Med Hyg
Autors principals: Cancino-Faure, Beatriz, Fisa, Roser, Alcover, M. Magdalena, Jimenez-Marco, Teresa, Riera, Cristina
Format: Artigo
Idioma:Inglês
Publicat: The American Society of Tropical Medicine and Hygiene 2016
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4889745/
https://ncbi.nlm.nih.gov/pubmed/27139452
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4269/ajtmh.15-0693
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