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PCR-Based Method Using Propidium Monoazide To Distinguish Viable from Nonviable Bacillus subtilis Spores

This paper describes a molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide. The approach should be valuable in our attempt to employ molecular methods to streamline the evaluation of p...

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Bibliografiske detaljer
Main Authors: Rawsthorne, H., Dock, C. N., Jaykus, L. A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2681676/
https://ncbi.nlm.nih.gov/pubmed/19270144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02524-08
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