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Cos-Seq for high-throughput identification of drug target and resistance mechanisms in the protozoan parasite Leishmania

Innovative strategies are needed to accelerate the identification of antimicrobial drug targets and resistance mechanisms. Here we develop a sensitive method, which we term Cosmid Sequencing (or “Cos-Seq”), based on functional cloning coupled to next-generation sequencing. Cos-Seq identified >60...

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Dettagli Bibliografici
Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Gazanion, Élodie, Fernández-Prada, Christopher, Papadopoulou, Barbara, Leprohon, Philippe, Ouellette, Marc
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4889358/
https://ncbi.nlm.nih.gov/pubmed/27162331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1520693113
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