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Cos-Seq for high-throughput identification of drug target and resistance mechanisms in the protozoan parasite Leishmania

Innovative strategies are needed to accelerate the identification of antimicrobial drug targets and resistance mechanisms. Here we develop a sensitive method, which we term Cosmid Sequencing (or “Cos-Seq”), based on functional cloning coupled to next-generation sequencing. Cos-Seq identified >60...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Main Authors: Gazanion, Élodie, Fernández-Prada, Christopher, Papadopoulou, Barbara, Leprohon, Philippe, Ouellette, Marc
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4889358/
https://ncbi.nlm.nih.gov/pubmed/27162331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1520693113
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