Detection of phosphorylated Akt and MAPK in cell culture assays

This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite fo...

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Detaylı Bibliyografya
Yayımlandı:MethodsX
Asıl Yazarlar: Molgaard, Simon, Ulrichsen, Maj, Olsen, Ditte, Glerup, Simon
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Elsevier 2016
Konular:
Biochemistry, Genetics and Molecular Biology
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4885140/
https://ncbi.nlm.nih.gov/pubmed/27274457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2016.04.009
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