Detection of phosphorylated Akt and MAPK in cell culture assays

This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite fo...

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Udgivet i:MethodsX
Main Authors: Molgaard, Simon, Ulrichsen, Maj, Olsen, Ditte, Glerup, Simon
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2016
Fag:
Biochemistry, Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4885140/
https://ncbi.nlm.nih.gov/pubmed/27274457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2016.04.009
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