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Detection of phosphorylated Akt and MAPK in cell culture assays
This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite fo...
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Publicado no: | MethodsX |
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Main Authors: | , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Elsevier
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4885140/ https://ncbi.nlm.nih.gov/pubmed/27274457 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2016.04.009 |
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