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Detection of phosphorylated Akt and MAPK in cell culture assays

This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite fo...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Molgaard, Simon, Ulrichsen, Maj, Olsen, Ditte, Glerup, Simon
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4885140/
https://ncbi.nlm.nih.gov/pubmed/27274457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2016.04.009
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