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Detection of phosphorylated Akt and MAPK in cell culture assays

This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite fo...

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Библиографические подробности
Опубликовано в: :MethodsX
Главные авторы: Molgaard, Simon, Ulrichsen, Maj, Olsen, Ditte, Glerup, Simon
Формат: Artigo
Язык:Inglês
Опубликовано: Elsevier 2016
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4885140/
https://ncbi.nlm.nih.gov/pubmed/27274457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2016.04.009
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