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Optimized expression and purification of biophysical quantities of the Lac repressor and Lac repressor regulatory domain
The recombinant production of Lac repressor (LacI) in E. coli is complicated by its ubiquitous use as a regulatory element in commercially-available expression vectors and host strains. While LacI-regulated expression systems are often used to produce recombinant LacI, the product can be heterogeneo...
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| Wydane w: | Protein Expr Purif |
|---|---|
| Główni autorzy: | , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
2016
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4881282/ https://ncbi.nlm.nih.gov/pubmed/27064119 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2016.04.003 |
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