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Optimized expression and purification of biophysical quantities of the Lac repressor and Lac repressor regulatory domain
The recombinant production of Lac repressor (LacI) in E. coli is complicated by its ubiquitous use as a regulatory element in commercially-available expression vectors and host strains. While LacI-regulated expression systems are often used to produce recombinant LacI, the product can be heterogeneo...
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| Publicado no: | Protein Expr Purif |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4881282/ https://ncbi.nlm.nih.gov/pubmed/27064119 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2016.04.003 |
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