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Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the functionality of genetic elements, creating genetically modified organisms as well as preclinical research of genetic disorders. However, the high frequency of off-target activity (≥50%)—RGEN (RNA-guided endonu...

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Detalhes bibliográficos
Publicado no:Mol Ther Nucleic Acids
Main Authors: Zhang, Xiao-Hui, Tee, Louis Y, Wang, Xiao-Gang, Huang, Qun-Shan, Yang, Shi-Hua
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4877446/
https://ncbi.nlm.nih.gov/pubmed/26575098
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mtna.2015.37
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