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Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia

BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug treatments. The complexity of human CNS means it un...

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Veröffentlicht in:BMC Psychiatry
Hauptverfasser: Dean, Brian, Udawela, Madhara, Scarr, Elizabeth
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2016
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4875643/
https://ncbi.nlm.nih.gov/pubmed/27206773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12888-016-0855-0
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