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Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia

BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug treatments. The complexity of human CNS means it un...

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Detalhes bibliográficos
Publicado no:BMC Psychiatry
Main Authors: Dean, Brian, Udawela, Madhara, Scarr, Elizabeth
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4875643/
https://ncbi.nlm.nih.gov/pubmed/27206773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12888-016-0855-0
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