Transfection of insect cell in suspension for efficient baculovirus generation

Baculovirus (BV) mediated insect cell expression system utilizes transfection as a first step to introduce recombinant baculovirus DNA into insect cells. Many labs are still relying on the conventional liposome based transfection method in adherent culture. Here we describe a more efficient method t...

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Detalles Bibliográficos
Publicado en:MethodsX
Main Authors: Roest, S., Kapps-Fouthier, S., Klopp, J., Rieffel, S., Gerhartz, B., Shrestha, B.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2016
Assuntos:
Biochemistry, Genetics and Molecular Biology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4872675/
https://ncbi.nlm.nih.gov/pubmed/27222826
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2016.04.011
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