A scalable suspension insect cell transfection method for production of baculoviruses with low amplification passages

Baculovirus expression vector systems (BEVS) have been widely used for production of recombinant proteins in insect cells. However, baculoviruses superinfection and repeated passages originate defective interfering particle (DIP) mutants, which is a limitation to a continuous large-scale production....

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Cabral, Aline Diniz, Garcia, Felipe Baena, da Costa, Renata Torres, Vasconcelos, Ligia Marinho Pereira, Uehara, Mabel, Santos, Edmar Silva, Sperança, Márcia Aparecida
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
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Protocol Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7586131/
https://ncbi.nlm.nih.gov/pubmed/33134099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2020.101103
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