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Development of a quantitative fluorescence-based ligand-binding assay

A major goal of biology is to develop a quantitative ligand-binding assay that does not involve the use of radioactivity. Existing fluorescence-based assays have a serious drawback due to fluorescence quenching that accompanies the binding of fluorescently-labeled ligands to their receptors. This li...

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Publicat a:Sci Rep
Autors principals: Breen, Conor J., Raverdeau, Mathilde, Voorheis, H. Paul
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 2016
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4861924/
https://ncbi.nlm.nih.gov/pubmed/27161290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep25769
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