Nucleosomes impede Cas9 access to DNA in vivo and in vitro

The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes. However, our understanding of how to select single-guide RNAs (sgRNAs) that mediate efficient Cas9 activit...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Horlbeck, Max A, Witkowsky, Lea B, Guglielmi, Benjamin, Replogle, Joseph M, Gilbert, Luke A, Villalta, Jacqueline E, Torigoe, Sharon E, Tjian, Robert, Weissman, Jonathan S
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2016
Assuntos:
Genes and Chromosomes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4861601/
https://ncbi.nlm.nih.gov/pubmed/26987018
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.12677
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