Interactions of liver Grp78 and Escherichia coli recombinant Grp78 with ATP: multiple species and disaggregation.

The hamster gene encoding the 78-kDa glucose-regulated protein (Grp78) was expressed in Escherichia coli as a fusion protein with glutathione S-transferase. After induction with isopropyl beta-D-thiogalactopyranoside, the recombinant Grp78 was purified to homogeneity by affinity column chromatograph...

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Bibliographic Details
Main Authors: Carlino, A, Toledo, H, Skaleris, D, DeLisio, R, Weissbach, H, Brot, N
Format: Artigo
Language:Inglês
Published: 1992
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC48600/
https://ncbi.nlm.nih.gov/pubmed/1532251
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