Interactions of liver Grp78 and Escherichia coli recombinant Grp78 with ATP: multiple species and disaggregation.

The hamster gene encoding the 78-kDa glucose-regulated protein (Grp78) was expressed in Escherichia coli as a fusion protein with glutathione S-transferase. After induction with isopropyl beta-D-thiogalactopyranoside, the recombinant Grp78 was purified to homogeneity by affinity column chromatograph...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Carlino, A, Toledo, H, Skaleris, D, DeLisio, R, Weissbach, H, Brot, N
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48600/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1532251/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.6.2081
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