A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy.
The bacteriophage P1 cloning system can package and propagate DNA inserts that are up to 95 kilobases. Clones are maintained in Escherichia coli by a low-copy replicon in the P1 cloning vector and can be amplified by inducing a second replicon in the vector with isopropyl beta-D-thiogalactopyranosid...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1992
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48595/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1549564/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.6.2056 |
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