A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy.

The bacteriophage P1 cloning system can package and propagate DNA inserts that are up to 95 kilobases. Clones are maintained in Escherichia coli by a low-copy replicon in the P1 cloning vector and can be amplified by inducing a second replicon in the vector with isopropyl beta-D-thiogalactopyranosid...

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Publicado en:	Proc Natl Acad Sci U S A
Principais autores:	Pierce, J C, Sauer, B, Sternberg, N
Formato:	Artigo
Idioma:	Inglês
Publicado:	National Academy of Sciences 1992
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48595/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1549564/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.6.2056
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