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Explant culture of adult zebrafish hearts for epicardial regeneration studies

Here we describe protocols to culture adult zebrafish hearts as explants, and to study regeneration of epicardial tissue ex vivo. Uninjured or injured adult hearts are excised, washed, and cultured in an incubator with gentle agitation. Preparation of heart explants is accomplished within 2 hours, a...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Nat Protoc
Main Authors: Cao, Jingli, Poss, Kenneth D.
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4857716/
https://ncbi.nlm.nih.gov/pubmed/27055096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.049
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