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Explant culture of adult zebrafish hearts for epicardial regeneration studies

Here we describe protocols to culture adult zebrafish hearts as explants, and to study regeneration of epicardial tissue ex vivo. Uninjured or injured adult hearts are excised, washed, and cultured in an incubator with gentle agitation. Preparation of heart explants is accomplished within 2 hours, a...

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Bibliografische gegevens
Gepubliceerd in:Nat Protoc
Hoofdauteurs: Cao, Jingli, Poss, Kenneth D.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2016
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4857716/
https://ncbi.nlm.nih.gov/pubmed/27055096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.049
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