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Explant culture of adult zebrafish hearts for epicardial regeneration studies
Here we describe protocols to culture adult zebrafish hearts as explants, and to study regeneration of epicardial tissue ex vivo. Uninjured or injured adult hearts are excised, washed, and cultured in an incubator with gentle agitation. Preparation of heart explants is accomplished within 2 hours, a...
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| Publié dans: | Nat Protoc |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2016
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4857716/ https://ncbi.nlm.nih.gov/pubmed/27055096 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.049 |
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