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Optimized P2A for reporter gene insertion into Nipah virus results in efficient ribosomal skipping and wild-type lethality

Incorporation of reporter genes within virus genomes is an indispensable tool for interrogation of virus biology and pathogenesis. In previous work, we incorporated a fluorophore into a viral ORF by attaching it to the viral gene via a P2A ribosomal skipping sequence. This recombinant Nipah virus, h...

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Vydáno v:J Gen Virol
Hlavní autoři: Park, Arnold, Yun, Tatyana, Hill, Terence E., Ikegami, Tetsuro, Juelich, Terry L., Smith, Jennifer K., Zhang, Lihong, Freiberg, Alexander N., Lee, Benhur
Médium: Artigo
Jazyk:Inglês
Vydáno: Microbiology Society 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4854364/
https://ncbi.nlm.nih.gov/pubmed/26781134
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1099/jgv.0.000405
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