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Selective amplification and sequencing of cyclic phosphate-containing RNAs by the cP-RNA-seq method
RNA digestions catalyzed by many ribonucleases generate RNA fragments containing a 2′,3′-cyclic phosphate (cP) at their 3′-termini. However, standard RNA-seq methods are unable to accurately capture cP-containing RNAs because the cP inhibits the adapter ligation reaction. We recently developed a met...
Shranjeno v:
| izdano v: | Nat Protoc |
|---|---|
| Main Authors: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2016
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4852555/ https://ncbi.nlm.nih.gov/pubmed/26866791 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.025 |
| Oznake: |
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