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Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing
The CRISPR/Cas9 system has been developed as an easy-handle and multiplexable approach for engineering eukaryotic genomes by zygote microinjection of Cas9 and sgRNA, while preparing Cas9 for microinjection is laborious and introducing inconsistency into the experiment. Here, we describe a modified s...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4847922/ https://ncbi.nlm.nih.gov/pubmed/27119535 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0154364 |
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