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Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi)
Clustered regularly interspersed short palindromic repeats (CRISPR) interference (CRISPRi) is a powerful technology for sequence-specifically repressing gene expression in bacterial cells. CRISPRi requires only a single protein and a custom-designed guide RNA for specific gene targeting. In Escheric...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4845913/ https://ncbi.nlm.nih.gov/pubmed/25981485 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2687-9_23 |
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