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Robust and stable transcriptional repression in Giardia using CRISPRi
Giardia lamblia is a binucleate protistan parasite causing significant diarrheal disease worldwide. An inability to target Cas9 to both nuclei, combined with the lack of nonhomologous end joining and markers for positive selection, has stalled the adaptation of CRISPR/Cas9-mediated genetic tools for...
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| Udgivet i: | Mol Biol Cell |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The American Society for Cell Biology
2019
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6337905/ https://ncbi.nlm.nih.gov/pubmed/30379614 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E18-09-0605 |
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