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Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors
Engineering the cofactor specificity of a natural enzyme often results in a significant decrease in its activity on original cofactor. Here we report that a NADH-dependent dehydrogenase (d-LDH) from Lactobacillus delbrueckii 11842 can be rationally engineered to efficiently use both NADH and NADPH a...
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| Publicat a: | Sci Rep |
|---|---|
| Autors principals: | , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4842997/ https://ncbi.nlm.nih.gov/pubmed/27109778 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep24887 |
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