A strategy for the generation of conditional mutations by protein destabilization.
Conditional mutations such as temperature-sensitive (ts) mutations are important for the analysis of protein function but are often difficult, or impossible, to obtain. Here we present a simple method for generating conditional mutations based on the use of a protein-destabilizing genetic element in...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1992
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48426/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1311090/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.4.1249 |
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