In vitro selection of restriction endonucleases by in vitro compartmentalization

Restriction endonucleases are widely used in laboratory applications from recombinant DNA technology to diagnostics, but engineering of restriction enzymes by structure-guided design and in vivo directed evolution is at an early stage. Here, we report the use of an in vitro compartmentalization syst...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Doi, Nobuhide, Kumadaki, Shin, Oishi, Yuko, Matsumura, Nobutaka, Yanagawa, Hiroshi
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2004
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC484195/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15247328/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh096
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