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In vitro selection of restriction endonucleases by in vitro compartmentalization

Restriction endonucleases are widely used in laboratory applications from recombinant DNA technology to diagnostics, but engineering of restriction enzymes by structure-guided design and in vivo directed evolution is at an early stage. Here, we report the use of an in vitro compartmentalization syst...

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Main Authors: Doi, Nobuhide, Kumadaki, Shin, Oishi, Yuko, Matsumura, Nobutaka, Yanagawa, Hiroshi
格式: Artigo
語言:Inglês
出版: Oxford University Press 2004
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC484195/
https://ncbi.nlm.nih.gov/pubmed/15247328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh096
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