Effects of different DNA polymerases in ligation-mediated PCR: enhanced genomic sequencing and in vivo footprinting.

We have developed a simplified procedure for the ligation-mediated polymerase chain reaction (LMPCR) using Thermococcus litoralis DNA polymerase (Vent DNA polymerase). We show that Vent DNA polymerase produces correct, blunt-ended primer extension products with substantially higher efficiency than T...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Garrity, P A, Wold, B J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48377/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1736283/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.3.1021
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