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Effects of different DNA polymerases in ligation-mediated PCR: enhanced genomic sequencing and in vivo footprinting.

We have developed a simplified procedure for the ligation-mediated polymerase chain reaction (LMPCR) using Thermococcus litoralis DNA polymerase (Vent DNA polymerase). We show that Vent DNA polymerase produces correct, blunt-ended primer extension products with substantially higher efficiency than T...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Garrity, P A, Wold, B J
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1992
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC48377/
https://ncbi.nlm.nih.gov/pubmed/1736283
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