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Effects of different DNA polymerases in ligation-mediated PCR: enhanced genomic sequencing and in vivo footprinting.

We have developed a simplified procedure for the ligation-mediated polymerase chain reaction (LMPCR) using Thermococcus litoralis DNA polymerase (Vent DNA polymerase). We show that Vent DNA polymerase produces correct, blunt-ended primer extension products with substantially higher efficiency than T...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Garrity, P A, Wold, B J
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48377/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1736283/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.3.1021
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