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High Throughput Measurement of Binding Kinetics via mRNA Display and Next-Generation Sequencing
There is great demand for high-throughput methods to characterize ligand affinity. By combining mRNA display with next-generation sequencing, we determined the kinetic on- and off-rates for over twenty thousand ligands, without the need for synthesis or purification of individual members. Our result...
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| Publicado no: | Angew Chem Int Ed Engl |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4834215/ https://ncbi.nlm.nih.gov/pubmed/26914638 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201600077 |
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