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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

One of the major questions in microbial ecology is “who is there?” This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA. Traditionally,...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Sanschagrin, Sylvie, Yergeau, Etienne
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828026/
https://ncbi.nlm.nih.gov/pubmed/25226019
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51709
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