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Analysis of 16S rRNA Amplicon Sequencing Options on the Roche/454 Next-Generation Titanium Sequencing Platform

BACKGROUND: 16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the out...

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מידע ביבליוגרפי
Main Authors: Tamaki, Hideyuki, Wright, Chris L., Li, Xiangzhen, Lin, Qiaoyan, Hwang, Chiachi, Wang, Shiping, Thimmapuram, Jyothi, Kamagata, Yoichi, Liu, Wen-Tso
פורמט: Artigo
שפה:Inglês
יצא לאור: Public Library of Science 2011
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3179495/
https://ncbi.nlm.nih.gov/pubmed/21966473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0025263
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