Analysis of 16S rRNA Amplicon Sequencing Options on the Roche/454 Next-Generation Titanium Sequencing Platform

BACKGROUND: 16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the out...

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Main Authors: Tamaki, Hideyuki, Wright, Chris L., Li, Xiangzhen, Lin, Qiaoyan, Hwang, Chiachi, Wang, Shiping, Thimmapuram, Jyothi, Kamagata, Yoichi, Liu, Wen-Tso
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3179495/
https://ncbi.nlm.nih.gov/pubmed/21966473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0025263
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