Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers. The overall coverage and phylum spectrum of 175 primers and 512 primer pairs w...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
Main Authors: Klindworth, Anna, Pruesse, Elmar, Schweer, Timmy, Peplies, Jörg, Quast, Christian, Horn, Matthias, Glöckner, Frank Oliver
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2013
Teme:
Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592464/
https://ncbi.nlm.nih.gov/pubmed/22933715
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks808
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki