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Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Macrophages, as key players of the innate immune response, are at the focus of research dealing with tissue homeostasis or various pathologies. Transfection with siRNA and plasmid DNA is an efficient tool for studying their function, but transfection of macrophages is not a trivial matter. Although...

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Pubblicato in:J Vis Exp
Autori principali: Maeß, Marten B., Wittig, Berith, Lorkowski, Stefan
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828023/
https://ncbi.nlm.nih.gov/pubmed/25226503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51960
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