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Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Macrophages, as key players of the innate immune response, are at the focus of research dealing with tissue homeostasis or various pathologies. Transfection with siRNA and plasmid DNA is an efficient tool for studying their function, but transfection of macrophages is not a trivial matter. Although...

Полное описание

Сохранить в:
Библиографические подробности
Опубликовано в: :J Vis Exp
Главные авторы: Maeß, Marten B., Wittig, Berith, Lorkowski, Stefan
Формат: Artigo
Язык:Inglês
Опубликовано: MyJove Corporation 2014
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828023/
https://ncbi.nlm.nih.gov/pubmed/25226503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51960
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