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Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Macrophages, as key players of the innate immune response, are at the focus of research dealing with tissue homeostasis or various pathologies. Transfection with siRNA and plasmid DNA is an efficient tool for studying their function, but transfection of macrophages is not a trivial matter. Although...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Maeß, Marten B., Wittig, Berith, Lorkowski, Stefan
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828023/
https://ncbi.nlm.nih.gov/pubmed/25226503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51960
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