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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

The paper describes the combination of optical tweezers and single molecule fluorescence detection for the study of protein-DNA interaction. The method offers the opportunity of investigating interactions occurring in solution (thus avoiding problems due to closeby surfaces as in other single molecu...

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Podrobná bibliografie
Vydáno v:J Vis Exp
Hlavní autoři: Monico, Carina, Belcastro, Gionata, Vanzi, Francesco, Pavone, Francesco S., Capitanio, Marco
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828021/
https://ncbi.nlm.nih.gov/pubmed/25226304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51446
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