Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates

BACKGROUND: Accurate adjustment for the amplification efficiency (AE) is an important part of real-time quantitative polymerase chain reaction (qPCR) experiments. The most commonly used correction strategy is to estimate the AE by dilution experiments and use this as a plug-in when efficiency correc...

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Publicat a:BMC Bioinformatics
Autors principals: Bilgrau, Anders E., Falgreen, Steffen, Petersen, Anders, Kjeldsen, Malene K., Bødker, Julie S., Johnsen, Hans E., Dybkær, Karen, Bøgsted, Martin
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2016
Matèries:
Methodology Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4827196/
https://ncbi.nlm.nih.gov/pubmed/27067838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-016-0997-6
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