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iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications
Using random mutagenesis and high throughput screening by microfluidic-assisted In Vitro Compartmentalization, we report the isolation of an order of magnitude times brighter mutants of the light-up RNA aptamers Spinach that are far less salt-sensitive and with a much higher thermal stability than t...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2016
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4824111/ https://ncbi.nlm.nih.gov/pubmed/26932363 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw083 |
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