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iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications

Using random mutagenesis and high throughput screening by microfluidic-assisted In Vitro Compartmentalization, we report the isolation of an order of magnitude times brighter mutants of the light-up RNA aptamers Spinach that are far less salt-sensitive and with a much higher thermal stability than t...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nucleic Acids Res
Prif Awduron: Autour, Alexis, Westhof, Eric, Ryckelynck, Michael
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2016
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4824111/
https://ncbi.nlm.nih.gov/pubmed/26932363
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw083
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