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Improved methods of AAV-mediated gene targeting for human cell lines using ribosome-skipping 2A peptide
The adeno-associated virus (AAV)-based targeting vector has been one of the tools commonly used for genome modification in human cell lines. It allows for relatively efficient gene targeting associated with 1–4-log higher ratios of homologous-to-random integration of targeting vectors (H/R ratios) t...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2016
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4824082/ https://ncbi.nlm.nih.gov/pubmed/26657635 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1338 |
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