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Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection
BACKGROUND: Targeted genomic editing using the CRISPR/Cas9 methodology has opened exciting new avenues in probing gene function in virtually any model system, including cultured mammalian cells. Depending on the desired mutation, several experimental options exist in the isolation of clonal lines, s...
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| Publicado en: | BMC Mol Biol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
BioMed Central
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4818936/ https://ncbi.nlm.nih.gov/pubmed/27038923 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12867-016-0061-0 |
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