Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos

BACKGROUND: The RNA guided CRISPR/Cas9 nucleases have been proven to be effective for gene disruption in various animal models including Xenopus tropicalis. The neural crest (NC) is a transient cell population during embryonic development and contributes to a large variety of tissues. Currently, los...

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Veröffentlicht in:Cell Biosci
Hauptverfasser: Liu, Zhongzhen, Cheng, Tina Tsz Kwan, Shi, Zhaoying, Liu, Ziran, Lei, Yong, Wang, Chengdong, Shi, Weili, Chen, Xiongfeng, Qi, Xufeng, Cai, Dongqing, Feng, Bo, Deng, Yi, Chen, Yonglong, Zhao, Hui
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2016
Schlagworte:
Research
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4818404/
https://ncbi.nlm.nih.gov/pubmed/27042291
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13578-016-0088-4
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