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Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility

Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycan...

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Bibliografske podrobnosti
izdano v:Methods Mol Biol
Main Authors: Bitto, David, Harvey, David J., Halldorsson, Steinar, Doores, Katie J., Pritchard, Laura K., Huiskonen, Juha T., Bowden, Thomas A., Crispin, Max
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4817836/
https://ncbi.nlm.nih.gov/pubmed/26169737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2874-3_7
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