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Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Enveloped viruses utilize membrane glycoproteins on their surface to mediate entry into host cells. Three-dimensional structural analysis of these glycoprotein ‘spikes’ is often technically challenging but important for understanding viral pathogenesis and in drug design. Here, a protocol is present...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Huiskonen, Juha T., Parsy, Marie-Laure, Li, Sai, Bitto, David, Renner, Max, Bowden, Thomas A.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4353292/
https://ncbi.nlm.nih.gov/pubmed/25350719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51714
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